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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 49.2 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human PD-L2 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) was greater than 90% as determined by SEC-HPLC.
Loaded Human PD-1, His Tag (Cat. No. PD1-H5221) on HIS1K Biosensor, can bind Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Human PD-1 Protein, His Tag (Cat. No. PD1-H522a) on HIS1K Biosensor, can bind Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) with an affinity constant of 15.7 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) on Protein A Biosensor, can bind Human PD-1 Protein, His Tag (Cat. No. PD1-H522a) with an affinity constant of 0.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Flow Cytometry assay shows that recombinant Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 1 μg/mL (Routinely tested).
Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L2 Protein, Fc Tag (Cat. No. PD2-H5251) with an EC50 of 0.018 μg/mL (Routinely tested).
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