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Flow cytometric analysis of Anti-MSLN CAR-293 cells staining with Alexa Fluor™ 647-Labeled Monoclonal Anti-G4S Linker Antibody, Rabbit IgG (016) (Cat. No. G4S-ABFYP1) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. Alexa Fluor 647 signal was used to evaluate the binding activity (QC tested).
Non-specificity of Alexa Fluor™ 647-Labeled Monoclonal Anti-G4S Linker Antibody, Rabbit IgG (016) (Cat. No. G4S-ABFYP1) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and Alexa Fluor™ 647-Labeled Monoclonal Anti-G4S Linker Antibody (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and Alexa Fluor 647 positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Immobilized Human anti-PD1×CTLA4 at 1 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-BLM25G0) with a linear range of 1.9-32 ng/mL (Routinely tested).
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