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Your Position: Home > Solutions for COVID-19 vaccine safety and immunogenicity evaluation

Solutions for COVID-19 vaccine safety and immunogenicity evaluation

Solutions for COVID-19 vaccine safety and immunogenicity evaluation
Background

With the efforts of the scientist around the world, up until now, there are now several COVID-19 vaccines that are in use.

In the development of a COVID-19 vaccine, safety and immunogenicity evaluation are indispensable and well-established assays are required for fast and convenient assessment of the vaccine candidates.

Based on advanced technology platform in recombinant protein production, ACROBiosystems has developed a series of critical reagents for COVID-19 vaccine development and evaluation, including ELISA kits, recombinant proteins and antibodies. These featured products are suitable for IgG/M antibody titer detection, neutralizing antibody titer detection and antigen titer detection to accelerate the vaccine development.

Applications and products
IgG antibody titer detection
>  Product: Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Spike RBD)
RAS-T024

Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 titer by Indirect-ELISA Assay. Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:400 human serum. Detection was performed using HRP-Anti-human IgG antibody with sensitivity of 98 ng/mL

>   Product: SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified)
The purity, molecular weight and polymeric form were rigorously verified by SDS-PAGE, SEC-MALS and NS-EM.

Fig 1. The purity of SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) was more than 90% verified by SDS-PAGE under reducing (R) condition. The molecular weight was around 550-660 kDa confirmed by SEC-MALS. The particles are similar in size and appearance to SARS-CoV-2 trimers reported in published literature verified by negative stain electron micrography.

Bioactivity of binding to anti-SARS-CoV-2 neutralizing antibody was verified by ELISA

Fig 2. Immobilized Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) with a linear range of 0.3-10 ng/mL

>   Product: Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His, Avitag™ (MALS verified)
Bioactivity of binding to anti-SARS-CoV-2 neutralizing antibody was verified by ELISA

Fig 3. Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL

Case Study:

Pfizer employed ACROBiosystems' featured biotinylated S protein RBD in the RBD-binding IgG assay during the phase 1/2 research. The interim report showed that the biotinylated S protein RBD from ACROBiosystems (Cat.No. SPD-C82E9)was bound to streptavidin-coated Luminex microspheres.[1]

Phase 1/2 Study to Describe the Safety and Immunogenicity of a COVID-19 RNA Vaccine Candidate (BNT162b1) in Adults 18 to 55 Years of Age: Interim Report. medRxiv  preprint. doi:  https://www.medrxiv.org/content/10.1101/2020.06.30.20142570v1
Neutralizing antibody titer detection
>  Product: Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit

Correlation analysis of RAS-N022 and FDA approved ELISA kit (detection of neutralizing antibody in post-vaccination serum)

>   Product: SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified)
Bioactivity of binding to anti-SARS-CoV-2 neutralizing antibody was verified by ELISA

Fig 5. Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.1-3 ng/mL

Bioactivity of binding to ACE-2 was verified by ELISA

Fig 6. Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-3 ng/mL

Antigen titer detection
>   Product: SARS-CoV-2 Spike Protein Titer Assay Kit

Fig 8. This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike protein. The kit consists of pre-coated Anti-SARS-CoV-2 Spike Protein Antibody Microplate, SARS-CoV-2 Spike protein as Control, biotin-Anti-SARS-CoV-2 Spike protein antibody and HRP-Streptavidin. Detection is performed using HRP-Streptavidin with sensitivity of 200 pg/mL.

Antibody isotype assay products
> Anti-SARS-CoV-2 IgG1/ IgG 2/ IgG 3/ IgG 4 Titer Serologic Assay Kit (S Protein RBD)

Fig.4 cross-validation data for antibody subtype detection

>>> related article:SARS-CoV-2 Infection: Do You Know About the Antibody-Dependent Enhancement (ADE)?

Product list
Proteins
MoleculeCat. No.Product Description
ACE2 proteinAC2-H52H8Human ACE2 / ACEH Protein, His Tag (MALS verified)
AC2-H82E6Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified)
AC2-H5257Human ACE2 / ACEH Protein, Fc Tag (MALS verified)
AC2-H82F9Biotinylated Human ACE2 / ACEH Protein, Fc,Avitag™
S trimer proteinSPN-C52H9SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified)
SPN-C82E9Biotinylated SARS-CoV-2 S protein, His,Avitag™, Super stable trimer (MALS verified)
SPN-C52H3SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified)
SPN-C82E3Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag™, Super stable trimer (MALS verified)
S protein RBDSPD-C52H3SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified)
SPD-C82E9Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)
SPD-C5259SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag(MALS verified)
SPD-C82AaBiotinylated SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified)
S1 proteinS1N-C52H4SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified)
S1N-C82E8Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His,Avitag™ (MALS verified)
S1N-C5256SARS-CoV-2 (COVID-19) S1 protein (D614G), His Tag
S1N-C82E3Biotinylated SARS-CoV-2 (COVID-19) S1 protein (D614G), His,Avitag™
S1 protein NTDS1D-C52H6SARS-CoV-2 (COVID-19) S1 protein NTD, His Tag
S1 protein CTDS1D-C52H3SARS-CoV-2 (COVID-19) S1 protein CTD, His Tag (MALS-verified)
S2 proteinS2N-C52H5SARS-CoV-2 (COVID-19) S2 protein, His Tag
Nucleocapsid proteinNUN-C5227SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag
NUN-C82E8Biotinylated SARS-CoV-2 (COVID-19) Nucleocapsid protein, His,Avitag™
Kits
  • Antibody titer assay kits

  • Antigen titer assay kits

  • Inhibitor screening kits and more

CategorySpeciesCat.No.Product DescriptionsamplePreorder/Order
Neutralizing antibody titer assay kit
SARS-CoV-2
Wild Type
RAS-N022Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay KitHuman Serum

Preorder

SARS-CoV-2
Alpha | B.1.1.7
(U.K.)
RAS-N028Anti-SARS-CoV-2 (B.1.1.7) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)Human Serum

Order

SARS-CoV-2
Beta | B.1.351
(South Africa)
RAS-N031Anti-SARS-CoV-2 (B.1.351) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)Human Serum

Order

SARS-CoV-2
Gamma | P.1
(Brazil)
RAS-N034Anti-SARS-CoV-2 (P.1) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)Human Serum

Order

SARS-CoV-2
Delta | B.1.617.2
(India)
RAS-N040Anti-SARS-CoV-2 (B.1.617.2) Neutralizing Antibody Serologic Assay Kit (Spike RBD)Human Serum

Preorder

RAS-N041Anti-SARS-CoV-2 (B.1.617.2) Neutralizing Antibody Serologic Assay Kit (Spike Trimer)Human Serum

Preorder

Antibody titer assay kit
SARS-CoV-2
Wild Type
RAS-T024Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD)Human Serum

Preorder

RAS-T025Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)Human Serum

Preorder

RAS-T001Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike S1)Human Serum

Order

RAS-T008Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Nucleocapsid Protein)Human Serum

Order

RAS-T009Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD)Human Serum

Order

RAS-T012Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay Kit (Spike S1)Human Serum

Order

RAS-T013Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay Kit (Spike RBD)Human Serum

Order

RAS-T018Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kitMouse Serum

Order

RAS-T023Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)Mouse Serum

Order

RAS-T019Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kitMonkey Serum

Order

SARS-CoV-2
Alpha | B.1.1.7
(U.K.)
RAS-T026Anti-SARS-CoV-2 (B.1.1.7) Antibody IgG Titer Serologic Assay Kit (Spike RBD)Human Serum

Order

RAS-T027Anti-SARS-CoV-2 (B.1.1.7) Antibody IgG Titer Serologic Assay Kit (Spike Protein)Human Serum

Order

SARS-CoV-2
Beta | B.1.351
(South Africa)
RAS-T029Anti-SARS-CoV-2 (B.1.351) Antibody IgG Titer Serologic Assay Kit (Spike RBD)Human Serum

Order

RAS-T030Anti-SARS-CoV-2 (B.1.351) Antibody IgG Titer Serologic Assay Kit (Spike Protein)Human Serum

Order

SARS-CoV-2
Gamma | P.1
(Brazil)
RAS-T032Anti-SARS-CoV-2 (P.1) Antibody IgG Titer Serologic Assay Kit (Spike RBD)Human Serum

Order

RAS-T033Anti-SARS-CoV-2 (P.1) Antibody IgG Titer Serologic Assay Kit (Spike Protein)Human Serum

Order

Antibody isotype assay kit
SARS-CoV-2
Wild Type
RAS-T014Anti-SARS-CoV-2 Antibody IgG1 Titer Serologic Assay kitHuman Serum

Order

RAS-T015Anti-SARS-CoV-2 Antibody IgG2 Titer Serologic Assay kitHuman Serum

Order

RAS-T016Anti-SARS-CoV-2 Antibody IgG3 Titer Serologic Assay kitHuman Serum

Order

RAS-T017Anti-SARS-CoV-2 Antibody IgG4 Titer Serologic Assay kitHuman Serum

Order

Cat.No.Product DescriptionsamplePreorder/Order
RAS-A010SARS-CoV-2 Nucleocapsid Protein Titer Assay KitVaccine samples

Order

RAS-A020SARS-CoV-2 Spike Protein Titer Assay KitVaccine samples

Order

RAS-A021SARS-CoV-2 Spike RBD Titer Assay KitVaccine samples

Order

LineageCat.No.Product DescriptionsamplePreorder/Order
SARS-CoV-2
(WT)
EP-105SARS-CoV-2 (COVID-19) Inhibitor screening Kitanti-viral drug

Order

EP-107SARS-CoV-2 (WT) Inhibitor Screening Kit (Spike RBD)anti-viral drug

Preorder

SARS-CoV-2
Alpha | B.1.1.7
(U.K.)
EP-108SARS-CoV-2 (B.1.1.7) Inhibitor Screening Kit (Spike RBD)anti-viral drug

Preorder

SARS-CoV-2
Beta | B.1.351
(South Africa)
EP-109SARS-CoV-2 (B.1.351) Inhibitor Screening Kit (Spike RBD)anti-viral drug

Preorder

SARS-CoV-2
Gamma | P.1
(Brazil)
EP-110SARS-CoV-2 (P.1) Inhibitor Screening Kit (Spike RBD)anti-viral drug

Preorder

SARS-CoV-2
Delta | B.1.617.2
(India)
EP-111SARS-CoV-2 (B.1.617.2) Inhibitor Screening Kit (Spike RBD)anti-viral drug

Preorder


SP-12SARS-CoV-2 Spike S1 protein-Coated Plates, Clear, 96-WellHuman Serum

Order


RP-13SARS-CoV-2 Spike protein RBD-Coated Plates, Clear, 96-WellHuman Serum

Order

Reference:
[1] Mulligan et al., (2020). Phase 1/2 Study to Describe the Safety and Immunogenicity of a COVID-19 RNA Vaccine Candidate (BNT162b1) in Adults 18 to 55 Years of Age: Interim Report. medRxiv, doi: https://doi.org/10.1101/2020.06.30.20142570 )

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