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> MHC Complex Custom Service 
Heterodimer co-expression for a more natural conformation with validated binding to W6/32 antibodies
Optional FITC/PE/APC/Biotin-labels available
MHC Class I and other antigens available
Monomer/Tetramer forms offered
Eukaryotic-based expression platform for eukaryotic protein conformation
Professional MHC-peptide complex R&D technology platform
Mature bioactivity verification: SPR/ELISA/FACS and more
| MHC Allele | Target |
|
|---|---|---|
| HLA-A*0101 HLA-A*0201 HLA-A*0301 HLA-A*1101 HLA-A*2402 HLA-A*30:01HLA-A*3303 HLA-B*1501 HLA-B*1525 HLA-B*3802 HLA-B*4601 HLA-B*0702 HLA-C*0102HLA-C*0303HLA-C*07:02:01HLA-DQA1*03:02&DQB1*03:03…… | NY-ESO-1 GP100 MSLN AFP WT-1 MAGE HPV HBV HIV EBVRYRCMVRHAMM-R3Glycipan 3 KRASEWHER2P53PRAMEPSMA…… |  |
Bioactivity
Homogeneity
Stability
Competitive Data
PE-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein (Cat. No. HL1-HP2E5) and negative control protein were first diluted in a 1:25 dilution ratio with FACS buffer. Staining was performed using 100 µL of working solution to 5e5 anti-NY-ESO-1 TCR-293 cells. Binding activity was evaluated based on PE signal intensity (QC tested).
PE-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer Protein (Cat. No. HL1-HP2E6) and negative control protein were first diluted in a 1:25 dilution ratio with FACS buffer. Staining was performed using 100 µL of working solution to 5e5 anti-NY-ESO-1 TCR-293 cells. Binding activity was evaluated based on PE signal intensity (QC tested).
Immobilized Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Complex Protein (Cat. No. HL1-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-NY-ESO-1 Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL (QC tested).
Immobilized Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Complex Protein (Cat. No. HL1-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-1 ng/mL (Routinely tested).
Anti-HLA class I Antibody, Human IgG1 (W6/32) captured on Protein A Chip can bind Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex Protein (Cat. No. HLD-H52H4) with an affinity constant of 1.06 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Performance of PE-labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer from ACROBiosystems (Cat. No. HL1-HP2E5) and other brand were evaluated by flow cytometry under the manufacturer’s recommended conditions. ACROBiosystems’ PE-Labeled NY-ESO-1 Tetramer Protein shows 3x higher MFI signal strength than competitor M, revealing much better TCR binding.
Performance of PE-labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer from ACROBiosystems (Cat. No. HL1-HP2E6) and other brand were evaluated by flow cytometry under the manufacturer’s recommended conditions. ACROBiosystems’ PE-Labeled NY-ESO-1 Tetramer Protein shows 2x higher MFI signal strength than competitor M, revealing much better TCR binding.
| Molecule | Cat. No. | Species | Product Description | Preorder/Order |
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