Integrin Proteins

Integrins are non-covalently-linked, heterodimeric molecules containing an α （120~185kD）and a β （90~110kD）subunit. Both subunits are type I transmembrane proteins, containing large extracellular domains and mostly short cytoplasmic domains. As a group of cell-matrix adhesion molecules, integrins play important roles in cell survival, proliferation, motility, differentiation, and ensuring appropriate cell localization. Thus, it is predicted that integrins involve in both mechanical engagement of cell to extracellular matrix, and generation of cellular signals during cancer malignancies development.

ACROBiosystems has developed a comprehensive series of Integrin proteins expressed by HEK293, and Avitag^TM biotinylated proteins can added your drug development. The proteins are suitable for ELISA, SPR, BLI and Cell based assay. ACRO has verified the purity and activity of the Integrin proteins, and organized the experimental operations and parameters into the corresponding protocol. Protocols are offered for free, which can help you save the development cycle.

Integrin proteins expressed by HEK293

High Bioactivity

SPR：

Human MAdCAM-1, His Tag (Cat. No. MAM-H52H4) immobilized on CM5 Chip can bind Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W4) with an affinity constant of 0.227 μM as determined in a SPR assay (Biacore 8K) (routinely tested).

Protocol

BLI：

Loaded Human Laminin 511 E8, Fc Tag (Cat. No. LA8-H5283) on Protein A Biosensor, can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. MAM-H52H4) with an affinity constant of 6.65 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested)

Protocol

ELISA：

Immobilized Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W6) with a linear range of 0.039-0.625 μg/mL (QC tested)

Protocol

Immobilized Mouse Latent TGFB1, His Tag (Cat. No. TG1-M5245) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT6-M82W9) with a linear range of 1-20 ng/mL (QC tested)

Protocol

High batch-to-batch Consistency

Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Human ITGAV&ITGB1 Heterodimer Protein, His Tag & Tag Free (Cat. No. IT1-H52E1) with a linear range of 0.02-0.313 μg/mL.