Cytokine detection ELISA kits

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ClinMax™ Cytokine Detection ELISA Kits

Workflow

Features

Quick & Easy-to-use: 2 hour assay with only 7 steps required.

Consistent: Calibrated using NIBSC/WHO standards to ensure batch-to-batch consistency.

Strict Quality Control: High sensitivity, specificity, accuracy, linearity and inter/intra precision.

Validated Method: Comprehensive validation with available protocol and 24h professional technical support.

Minimal Matrix Interference: Low matrix interference verified in cell culture supernatant and serum.

Application

Product List

To meet the different requirements in early discovery and clinical trials, ClinMax™ offers well-qualified RUO and Pro reagents. Click to check the differences between RUO and PRO

	RUO		PRO
Molecule	Cat. No.	Product Description	Cat. No.	Product Description
IFN-γ	CRS-A017	ClinMax™ Human Interferon-γ(IFN-γ) ELISA Kit	CEA-C006	ClinMax™ Human IFN-γ ELISA Kit, PRO
TNF-α	CRS-A002	ClinMax™ Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit	CEA-C010	ClinMax™ Human TNF-α ELISA Kit, PRO
IL-6	CRS-B001	ClinMax™ Human IL-6 ELISA Kit	CEA-C001	ClinMax™ Human IL-6 ELISA Kit, PRO
IL-1 beta	CRS-B002	ClinMax™ Human IL-1beta ELISA Kit	CEA-C002	ClinMax™ Human IL-1β ELISA Kit, PRO
IL-4	CRS-B003	ClinMax™ Human IL-4 ELISA Kit	CEA-C003	ClinMax™ Human IL-4 ELISA Kit, PRO
IL-8	CRS-B004	ClinMax™ Human IL-8 ELISA Kit	CEA-C004	ClinMax™ Human IL-8 ELISA Kit, PRO
IL-10	CRS-B005	ClinMax™ Human IL-10 ELISA Kit	CEA-C005	ClinMax™ Human IL-10 ELISA Kit, PRO
GM-CSF	CRS-B007	ClinMax™ Human GM-CSF ELISA Kit	CEA-C007	ClinMax™ Human GM-CSF ELISA Kit, PRO
IL-2	CRS-B008	ClinMax™ Human Interleukin-2(IL-2) ELISA Kit	CEA-C008	ClinMax™ Human IL-2 ELISA Kit, PRO
IL-12 (P70)	CRS-B009	ClinMax™ Human IL-12p70 ELISA Kit	CEA-C009	ClinMax™ Human IL-12p70 ELISA Kit, PRO

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RUO & Pro , which is more suitable for your current application?

		RUO	PRO
Detection Time		2-3h	2h
Sample Type		Cell Culture Supernates, Serum , Plasma	Cell Culture Supernates, Serum , Plasma
Quality Management System		ISO13485
Validation Guidance			ICH M10
Validation Experiment	Hook effect	√	√
	Matrix effect		Bilirubin, Hemoglobin, Triglyceride
	Anticoagulant effect		EDTA, Heparin, Sodium citrate
	Influence of sample storage conditions		3 Freeze–thaw Cycles
	Specificity	IL-2, IL-4, IL-6, IL-7, IL-10, IL-15, IL-21, IL-1β, TNF-alpha, GM-CSF	IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 p70, IL-13, IL-15, IL-17, GM-CSF, TNF-alpha, IFN-gamma, etc.

Assay Data

RUO

ClinMax™ Human Interferon-γ (IFN-γ) ELISA Kit ( CRS-A017 )

Typical Data

The sensitivity of ClinMax™ Human Interferon-γ (IFN-γ) ELISA Kit (Cat. No. CRS-A017) is 5.309 pg/Ml.

Precision

ClinMax™ Human Interferon-γ (IFN-γ) ELISA Kit (Cat. No. CRS-A017) was verified to have an intra/inter-precision of less than 10%.

PRO

ClinMax™ Human IFN-γ ELISA Kit, Pro ( CEA-C006 )

Typical Data

The sensitivity of ClinMax™ Human IFN-γ ELISA Kit, PRO (Cat. No. CEA-C006) is 3.25 pg/mL.

Precision

ClinMax™ Human IFN-γ ELISA Kit, PRO (Cat. No. CEA-C006) was verified to have an intra/inter-precision of less than 10%.

Matrix and Anticoagulant Effect

Diseases that are commonly present during sample collection such as jaundice (elevated bilirubin), hemolysis (elevated hemoglobin), and lipemia (elevated triglyceride) were simulated. Bilirubin, hemoglobin, and triglycerides were individually spiked into the sample matrix at a concentration of 20 mg/dL, 3500 g/dL, 2.0 g/L, respectively to evaluate matrix effect on our (XXX) kits.

Anticoagulant effect was tested by spiking the sample with either heparin, EDTA, or sodium citrate at a concentration of 40U/mL, 4mg/mL, and 40mg/mL, respectively.

Application Cases

Applications: Use Cat. No. CRS-A017 to detect IFN- γ content in cell culture supernatan to evaluate the killiing ability of CAR-T cells After co-culturing CAR-T cells with target cells.

Standard Curve

IFN-γ Standard	OD1	OD2	AVERAGE	ODAVERAGE-BLANK
0pg/mL(Blank)	0.0583	0.0472	0.05275	0
19.5pg/mL	0.084	0.081	0.0825	0.02975
39.1pg/mL	0.1324	0.1403	0.13635	0.0836
78.1pg/mL	0.2442	0.2253	0.23475	0.182
156.25pg/mL	0.427	0.4125	0.41975	0.367
312.5pg/mL	0.7573	0.7332	0.74525	0.6925
625pg/mL	1.3271	1.3245	1.3258	1.27305
1250pg/mL	2.2102	2.1864	2.1983	2.14555

The results of testing cell supernatant samples from different groups

Samples	OD1	OD2	AVERAGE	ODAVERAGE-BLANK	IFN-γ content（pg/mL）
1	0.752	0.7561	0.75405	0.7013	313.853045
2	0.105	0.1045	0.10475	0.052	27.128759
3	0.092	0.0971	0.09455	0.0418	23.219143
4	0.0952	0.1003	0.09775	0.045	24.442757
5	0.1008	0.1026	0.1017	0.04895	25.956882
6	0.112	0.0994	0.1057	0.05295	27.494249
7	0.0994	0.0959	0.09765	0.0449	24.404478
8	0.3364	0.3455	0.34095	0.2882	123.398059
9	0.1995	0.1846	0.19205	0.1393	61.534605
10	0.136	0.1409	0.13845	0.0857	40.223079

To meet the broadly needs of cytokine detection based on ELISA method and solve its limitations such as long operation time and batch-to-batch difference, ACROBiosystems have specifically developed a series of ClinMax™ cytokine detection kits, aiming to provide a more reliable analytical reagent for drug discovery, quality control, preclinical study and clinical trials.

Ideal for research, optimal for clinical trials