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Your Position: Home > Star Staining Fluorescent-labeled Products New Generation Tools for CAR Detection

Star Staining Fluorescent-labeled Products
New Generation Tools for CAR Detection

Star Staining
Introduction
Star Staining fluorescent-labeled products are developed by a new-generation site-specific labeling technology with "Star Standard" quality at ACROBiosystems. These products are uniquely designed for detecting and monitoring CAR-T cells in clinical trials.
Fluorescent-labeled protein is a powerful tool for the detection of CAR expression in research and clinical samples by flow cytometry. These proteins are pre-labeled fluorescent dyes that can detect CAR expression by one-step staining with minimal background. However, the development of high-quality fluorescent-labeled proteins presents a major challenge and there are only few products commercially available so far.
Conjugation technique is a major bottleneck for developing high-quality fluorescent-labeled proteins. Currently, the most widely used labeling technique in the market is the traditional chemical labeling approach. It is a very easy method to covalently label protein with a fluorescent dye in a non-specific manner. However, random modification can lead to heterogeneous and may affect the bioactivity of the protein via blocking the protein-active site. Studies indicate that the new-generation site-specific labeling technology can attach fluorescent dyes to specific protein in site-specific manner. It provides a guarantee to the uniformity of the fluorescent-labeled products and avoids affecting protein activity. This property makes it a useful weapon for the development of high-quality fluorescent-labeled protein products with high specificity and sensitivity.
Product Feature
Traditional Chemical Labeling
Claudin18.2-VLP
Non-selective random labeling and affect natural structure
May cover the active sites to reduce bioactivity
Easy to aggregate due to change the hydrophilicity of proteins
High variation in batch-to-batch consistency
New-generation Site-specific Labeling
Nanodisc Platform
The labeling happens at the specific tag and far away from active sites of proteins
Maintain natural conformation and modification
Efficiently bio-orthogonal technology
High batch-to-batch consistency and uniformity.
Star Staining Features

ACROBiosystems is striving for developing the high-standard detection reagents to support the development of CAR-T cell therapy. After years of research and experience by our technicians, we successfully established the "Star Staining" site-specific labeling platform and developed a series of exclusive fluorescent-labeled proteins with "Star Standard" quality. "Star Staining" products can be the powerful tools for detecting and monitoring CAR-T cells in clinical trials.

Using new-generation site-specific labeling technology to maintain natural bioactivity

High specificity and sensitivity verified by flow cytometry

No non-specific binding to non-transduced PBMCs

High batch-to-batch consistency and uniformity

Hot Products
Fluorescent LabelsMoleculeCat.No.Product descriptionPreorder/Order
FITCBCMABCA-HF2H3FITC-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining

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CD19CD9-HF2H3FITC-Labeled Human CD19 (20-291) Protein, His Tag star staining

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MesothelinMSN-HF2H3FITC-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining

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PEBCMABCA-HP2H7PE-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining

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CD19CD9-HP2H5PE-Labeled Human CD19 (20-291) Protein, His Tag star staining

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MesothelinMSN-HP2H8PE-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining

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Siglec-2SI2-HP2H5PE-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining

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APCBCMABCA-HA2H4APC-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining

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CD19CD9-HA2H9APC-Labeled Human CD19 (20-291) Protein, His Tag star staining

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MesothelinMSN-HA2H6APC-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining

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More Star Staining Products
  • FITC

  • PE

  • APC

  • Alexa Fluor 647

  • Alexa Fluor 555

  • Alexa Fluor 488

Fluorescent LabelsMoleculeProduct descriptionPreorder/Order
FITCBCMAFITC-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining

Order

CD19FITC-Labeled Human CD19 (20-291) Protein, His Tag star staining

Order

MesothelinFITC-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining

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Glypican 3FITC-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining

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HER2FITC-Labeled Human Her2 / ErbB2 Protein, His Tag star staining

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CD7FITC-Labeled Human CD7 Protein, His Tag star staining

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Siglec-3FITC-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining

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CD30FITC-Labeled Human CD30 / TNFRSF8 Protein, His Tag star staining

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EGF RFITC-Labeled Human EGF R Protein, His Tag star staining

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EGFRVIIIFITC-Labeled Human EGFRvIII Protein, His Tag star staining

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GUCY2CFITC-Labeled Human GUCY2C / Guanylyl cyclase C Protein, His Tag star staining

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NKG2DFITC-Labeled Human NKG2D / CD314 Protein, His Tag star staining

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FAPFITC-Labeled Human FAP Protein, His Tag star staining

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Nectin-4FITC-Labeled Human Nectin-4 Protein, His Tag star staining

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CD37FITC-Labeled Human CD37 Protein, His Tag star staining

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protein LFITC-Labeled Recombinant Protein L Protein, His Tag star staining

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Fluorescent LabelsMoleculeProduct descriptionPreorder/Order
PEBCMAPE-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining

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CD19PE-Labeled Human CD19 (20-291) Protein, His Tag star staining

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MesothelinPE-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining

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Glypican 3PE-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining

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NKG2DPE-Labeled Human NKG2D / CD314 Protein, His Tag star staining

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FAPPE-Labeled Human FAP Protein, His Tag star staining

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Nectin-4PE-Labeled Human Nectin-4 Protein, His Tag star staining

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CD37PE-Labeled Human CD37 Protein,  His Tag star staining

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protein LPE-Labeled Recombinant Protein L Protein, His Tag star staining

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EMMPRINPE-Labeled Human EMMPRIN / CD147 Protein, His Tag star staining

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CD300ePE-Labeled Human CD300e Protein, His Tag star staining

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HER2PE-Labeled Human Her2 / ErbB2 Protein, His Tag star staining

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Siglec-2PE-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining

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Siglec-3PE-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining

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PD-1PE-Labeled Human PD-1 Protein, His Tag star staining

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OX40PE-Labeled Human OX40 Protein, His Tag star staining

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Mucin-1PE-Labeled Human MUC-1 Protein, His Tag star staining

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TSLP RPE-Labeled Human TSLP R Protein, His Tag star staining

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Fluorescent LabelsMoleculeProduct descriptionPreorder/Order
APCBCMAAPC-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining

Preorder

CD19APC-Labeled Human CD19 (20-291) Protein, His Tag star staining

Preorder

MesothelinAPC-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining

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Glypican 3APC-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining

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EMMPRINAPC-Labeled Human EMMPRIN / CD147 Protein, His Tag star staining

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CD300eAPC-Labeled Human CD300e Protein, His Tag star staining

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HER2APC-Labeled Human Her2 / ErbB2 Protein, His Tag star staining

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Siglec-2APC-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining

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Siglec-3APC-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining

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PD-1APC-Labeled Human PD-1 Protein, His Tag star staining

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OX40APC-Labeled Human OX40 Protein, His Tag star staining

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Mucin-1APC-Labeled Human Mucin-1/MUC-1 Protein, His Tag star staining

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TSLP RAPC-Labeled Human TSLP R Protein, His Tag star staining

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Fluorescent LabelsMoleculeProduct descriptionPreorder/Order
Alexa Fluor 647BCMAAlexa Fluor 647-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining

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CD19Alexa Fluor 647-Labeled Human CD19 (20-291) Protein, His Tag star staining

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MesothelinAlexa Fluor 647-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining

Preorder

Glypican 3Alexa Fluor 647-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining

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EMMPRINAlexa Fluor 647-Labeled Human EMMPRIN / CD147 Protein, His Tag star staining

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CD300eAlexa Fluor 647-Labeled Human CD300e Protein, His Tag star staining

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HER2Alexa Fluor 647-Labeled Human Her2 / ErbB2 Protein, His Tag star staining

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Siglec-2Alexa Fluor 647-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining

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Siglec-3Alexa Fluor 647-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining

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PD-1Alexa Fluor 647-Labeled Human PD-1 Protein, His Tag star staining

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OX40Alexa Fluor 647-Labeled Human OX40 Protein, His Tag star staining

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Mucin-1Alexa Fluor 647-Labeled Human Mucin-1/MUC-1 Protein, His Tag star staining

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TSLP RAlexa Fluor 647-Labeled Human TSLP R Protein, His Tag star staining

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Fluorescent LabelsMoleculeProduct descriptionPreorder/Order
Alexa Fluor 555BCMAAlexa Fluor 555-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining

Preorder

CD19Alexa Fluor 555-Labeled Human CD19 (20-291) Protein, His Tag star staining

Preorder

MesothelinAlexa Fluor 555-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining

Preorder

Glypican 3Alexa Fluor 555-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining

Preorder

NKG2DAlexa Fluor 555-Labeled Human NKG2D / CD314 Protein, His Tag star staining

Preorder

FAPAlexa Fluor 555-Labeled Human FAP Protein, His Tag star staining

Preorder

Nectin-4Alexa Fluor 555-Labeled Human Nectin-4 Protein, His Tag star staining

Preorder

CD37Alexa Fluor 555-Labeled Human CD37 Protein,  His Tag star staining

Preorder

protein LAlexa Fluor 555-Labeled Recombinant Protein L Protein, His Tag star staining

Preorder

Fluorescent LabelsMoleculeProduct descriptionPreorder/Order
Alexa Fluor 488BCMAAlexa Fluor 488-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining

Preorder

CD19Alexa Fluor 488-Labeled Human CD19 (20-291) Protein, His Tag star staining

Preorder

MesothelinAlexa Fluor 488-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining

Preorder

Glypican 3Alexa Fluor 488-Labeled Human Glypican 3 / GPC3 Protein, His Tag star staining

Preorder

EMMPRINAlexa Fluor 488-Labeled Human EMMPRIN / CD147 Protein, His Tag star staining

Preorder

CD300eAlexa Fluor 488-Labeled Human CD300e Protein, His Tag star staining

Preorder

HER2Alexa Fluor 488-Labeled Human Her2 / ErbB2 Protein, His Tag star staining

Preorder

Siglec-2Alexa Fluor 488-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining

Preorder

Siglec-3Alexa Fluor 488-Labeled Human Siglec-3 / CD33 Protein, His Tag star staining

Preorder

PD-1Alexa Fluor 488-Labeled Human PD-1 Protein, His Tag star staining

Preorder

OX40Alexa Fluor 488-Labeled Human OX40 Protein, His Tag star staining

Preorder

Mucin-1Alexa Fluor 488-Labeled Human Mucin-1/MUC-1 Protein, His Tag star staining

Preorder

TSLP RAlexa Fluor 488-Labeled Human TSLP R Protein, His Tag star staining

Preorder

Data Display

High purity

High purity than 90% of Star Staining FITC-labeled Human BCMA
BCMA

FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

BCMA

The purity of FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) is more than 90% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.

High bioactivity

Higher binding activity than that of other competitors
FITC-labeled
FITC-labeled

Binding activity of FITC-Labeled Human BCMA and CD19 protein from two different vendors were evaluated by the FACS analysis. The result showed that ACRO's Star Staining FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) and CD19 (Cat. No. CD9-HF2H3) protein have a much higher binding activity than that of the other competitor.

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Star Staining PE-CD19 or APC-CD19 protein can detect CD19 CAR expression with high sensitivity by flow cytometry.
Anti-CD19 CAR-293

Anti-CD19 CAR-293 cells were stained with PE-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HP2H5), His Tag star staining and negative control protein respectively, PE signal was used to evaluate the binding activity.

Anti-CD19 CAR-293

Anti-CD19 CAR-293 cells were stained with APC-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HA2H9), His Tag star staining and negative control protein respectively, APC signal was used to evaluate the binding activity.

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No non-specific binding to non-transduced PBMCs

Star Staining products have no non-specific binding to non-transduced PBMCs with higher binding activity than that of other competitors
FITC-labeled

Non-specific binding to non-transduced PBMCs between PE-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with PE -Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and PE signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.

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FITC-labeled

Non-specific binding to non-transduced PBMCs between FITC-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with FITC-Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and FITC signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.

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Maintain natural bioactivity

High binding capacity before and after conjugation, verified by FACS and SPR
FITC-labeled

Binding activity of the Human BCMA before and after FITC labeling was evaluated in the above FACS analysis. The result shows that FITC-Labeled BCMA (Cat. No. BCA-HF2H3) and unconjugated Human BCMA have almost the same level of binding activity.

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FITC-labeled
FITC-labeled

Binding affinity of the Human BCMA before and after FITC labeling was evaluated in the above SPR analysis (Biacore T200). The result shows that FITC-Labeled (Cat. No. BCA-HF2H3) and unconjugated Human BCMA, His Tag have almost the same level of affinity.

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High batch-to-batch consistency

Binding activity of different lots of FITC/Alexa Fluor-555/PE-labeled Human BCMA protein was verified by FACS
FITC-labeled
FITC-labeled
FITC-labeled

Binding activity of different lots of FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) , AF555-Labeled Human BCMA (Cat. No. BCA-HA2H6) and PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) against anti-BCMA CAR-293 cells was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.

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High stability

FITC-labeled
FITC-labeled

PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) and FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3)  25℃ for 48 hours and freeze-throw cycles are stable without performance reduction.

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More CAR-T Related Products

REFERENCES

[1] Abbasov ME, Kavanagh ME, Ichu TA, et al. A proteome-wide atlas of lysine-reactive chemistry [published online ahead of print, 2021 Sep 9]. Nat Chem. 2021;10.1038/s41557-021-00765-4.

[2] Xu L, Kuan SL, Weil T. Contemporary Approaches for Site-Selective Dual Functionalization of Proteins. Angew Chem Int Ed Engl. 2021;60(25):13757-13777.

[3] Devabhaktuni A, Lin S, Zhang L, et al. TagGraph reveals vast protein modification landscapes from large tandem mass spectrometry datasets. Nat Biotechnol. 2019;37(4):469-479.

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