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Your Position: Home > Kits > - > RIPO-RWM009K

Human iPSC-Derived Liver Organoid Differentiation Kit

For research use only.

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Materials Provided
Box A(2-8°C)
IDComponentsSize
RIPO-RWM009K-C01Liver organoid Basal medium A9ml
RIPO-RWM009K-C02Liver organoid Basal medium B9ml
RIPO-RWM009K-C03Liver organoid Basal medium C13.5ml
RIPO-RWM009K-C04Liver organoid Basal medium D22.5ml
 
Box B(-20°C)
IDComponentsSize
RIPO-RWM009K-1-C01Liver organoid Supplement A1ml
RIPO-RWM009K-1-C02Liver organoid Supplement B1ml
RIPO-RWM009K-1-C03Liver organoid Supplement C-11.2ml
RIPO-RWM009K-1-C04Liver organoid Supplement C-20.3ml
RIPO-RWM009K-1-C05Liver organoid Supplement D-12ml
RIPO-RWM009K-1-C06Liver organoid Supplement D-20.5ml
  • Product Details
    Human iPSC-Derived Liver Organoid Differentiation Kit allows hESC or hiPSC to differentiate into liver organoids. Liver organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative to the human liver. This kit can produce 48 liver organoids in four steps.
  • Product Specification
    The basic medium of this kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect organoid differentiation.
  • Storage

    The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
    The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
    The opened kit should be stored according to components table.

*Please note that to support full process of organoid culture and maintenance, this product have to be used with Human iPSC-Derived Liver Organoid Expansion and Maintenance Medium (Cat. No.RIPO-RWM010),you can follow this link for product information.
Product Diagram
 - PRODUCT DIAGRAM

Protocol Diagram of liver organoid differentiation.

Validation Data
Orgnaoid Histology
 - ORGNAOID HISTOLOGY

H&E Staining of liver organoid.

Marker Expression
 - MARKER EXPRESSION

The liver organoids show expression of hepatocytes (HFN4a), hepatobiliary tracts (CK19), Albumin (ALB) and vascular endothelial cell (CD31).

 - MARKER EXPRESSION

Pre-mature liver organoids show liver lobules-like structure.

Organoid Activity
 - ORGANOID ACTIVITY

Liver organoids show better performance than HepG2 cell line in terms of the expression of Albumin, CYP3A4, CYP1A2, CYP2C9, CYP7A1 and BSEP.

 - ORGANOID ACTIVITY

Liver organoid show response to Rifampicin treatment as indication by the increase fluorescence intensity of CYP3A4.

  • Clinical and Translational Updates

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Price(USD) : $650.00

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