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Flow Cytometry assay shows that Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 0.1 μg/mL (Routinely tested).
FACS analysis shows that the binding of Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5) to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing Anti-PD-1 antibody. The concentration of PD-L2 used is 0.1 μg/mL. The IC50 is 1.3 μg/mL (Routinely tested).
Captured Human PD-L2 Protein, His Tag (Cat. No. PD2-H5220) on CM5 Chip via anti-His antibody can bind Human PD-1, Fc Tag (Cat. No. PD1-H5257) with an affinity constant of 24.9 nM as determined in SPR assay (Biacore T200) (QC tested).
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