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Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
CD71
Mouse
Mouse IgG1 | Mouse Kappa
Unconjugated
Recombinant Monoclonal
Human
Recombinant Human CD71 is expressed from human 293 cells.
Specifically recognizes Human CD71.
| Application | Recommended Usage |
| ELISA | 0.1-63 ng/mL |
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
This product No cross-reactivity in ELISA with
Rat Transferrin R, His Tag (Cat. No. TFR-R5249).
Mouse Transferrin R, His Tag (Cat. No. TFR-M524b).
Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Immobilized Cynomolgus Transferrin R, His Tag (Cat. No. TFR-C524a) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) with a linear range of 0.1-8 ng/mL (QC tested).
Immobilized Human Transferrin R, His Tag (Cat. No. CD1-H5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) with a linear range of 0.1-4 ng/mL (QC tested).
Captured Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) on CM5 chip via anti-mouse antibodies surface can bind Human Transferrin R, His Tag (Cat. No. CD1-H5243) with an affinity constant of 0.821 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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