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This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 47.2 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human DR3 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) is more than 90% and the molecular weight of this protein is around 95-120 kDa verified by SEC-MALS.
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a linear range of 0.005-0.625 μg/mL (QC tested).
Immobilized Human TL1A Protein, His Tag (Cat. No. TLA-H5244) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a linear range of 0.02-0.625 μg/mL (Routinely tested).
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Monoclonal Anti-TL1A Antibody, Human IgG1 and 4 μg/mL (50 μL/well) Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a half maximal inhibitory concentration (lC50) of 1.976 μg/mL (Routinely tested).
Immobilized Human TL1A Protein, His Tag (Cat. No. TLA-H5244) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Monoclonal Anti-TL1A Antibody, Human IgG1 and 4 μg/mL (50 μL/well) Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a half maximal inhibitory concentration (lC50) of 3.06 μg/mL (Routinely tested).
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