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This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 42.0 kDa. The protein migrates as 57-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, Fc,Avitag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, Fc,Avitag (MALS verified) (Cat. No. CD7-C82F3) is more than 85% and the molecular weight of this protein is around 105-125 kDa verified by SEC-MALS.
Immobilized Cynomolgus SIRP alpha, Fc Tag (Cat. No. SIA-C5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, Fc,Avitag (MALS verified) (Cat. No. CD7-C82F3) with a linear range of 0.2-5 ng/mL (QC tested).
Immobilized Monoclonal Anti-Human CD47 Antibody, Human IgG4 at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CD47 Protein, Fc,Avitag (MALS verified) (Cat. No. CD7-C82F3) with a linear range of 0.1-3 ng/mL (Routinely tested).
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