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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 72.5 kDa. The protein migrates as 80-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human GUCY2C, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human GUCY2C, Fc Tag (Cat. No. GUC-H5257) is more than 90% and the molecular weight of this protein is around 190-210 kDa verified by SEC-MALS.
Immobilized Human GUCY2C, Fc Tag (Cat. No. GUC-H5257) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).
2e5 of anti-GUCY2C CAR-293 cells were stained with 100 µL of 1 µg/mL Human GUCY2C, Fc Tag (Cat. No. GUC-H5257) and negative control protein respectively, washed and then followed by PE anti-human IgG Fc and analyzed with FACS (Routinely tested).
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