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This protein carries a human IgG1 Fc tag at the N-terminus and an Avi tag (Avitag™) at the C-terminus
The protein has a calculated MW of 43.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human IL-2, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) with a linear range of 1-39 ng/mL (QC tested).
Immobilized Human IL-2 R alpha, Fc Tag (Cat. No. ILA-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) with a linear range of 0.6-10 ng/mL (Routinely tested).
Loaded Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) on SA Biosensor, can bind Human IL-2 R beta, His Tag (SPR verified) (Cat. No. CD2-H5221) with an affinity constant of 2.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) on SA Biosensor, can bind Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) with an affinity constant of 47.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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