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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.7 kDa. The protein migrates as 28-42 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 0.5M Arginine, PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Mouse CD47, His Tag (Cat. No. CD7-M522b) is more than 90% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.
Immobilized Mouse SIRP alpha Protein, Mouse IgG2a Fc Tag (Cat. No. SIA-M5252) at 10 μg/mL (100 μL/well) can bind Mouse CD47, His Tag (Cat. No. CD7-M522b) with a linear range of 0.078-5 μg/mL (QC tested).
Mouse SIRP alpha, His Tag (Cat. No. SIA-M52H4) immobilized on CM5 Chip can bind Mouse CD47, His Tag (Cat. No. CD7-M522b) with an affinity constant of 2.52 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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