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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 46.8 kDa. The protein migrates as 60-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human OX40, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human OX40, Fc Tag (Cat. No. OX0-H5255) is more than 85% and the molecular weight of this protein is around 110-140 kDa verified by SEC-MALS.
Immobilized Human OX40 Ligand, His Tag (active trimer) (Cat. No. OXL-H52Q8) at 5 μg/mL (100 μL/well) can bind Human OX40, Fc Tag (Cat. No. OX0-H5255) with a linear range of 0.4-3 ng/mL (QC tested).
Quantitative Analysis of CTLA-4 x OX40 Bispecific Antibody in Human Serum by Intact Assay.
Immobilized Human OX40, Fc Tag (Cat. No. OX0-H5255) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 2 ng/mL (Intact assay, Routinely tested).
FACS assay shows that recombinant Human OX40, Fc Tag (Cat. No. OX0-H5255) can bind to 293T cell overexpressing human OX40L. The concentration of OX40 used is 0.03 μg/ml (Routinely tested).
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