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50x Digitonin buffer

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  • Composition
    This 50x detergent solution contains 4%(w/v) Digitonin that is necessary for solubilization of membrane proteins. Digitonin [CAS: 11024-24-1], is a non-ionic detergent that provide a more membrane-like environment for solubilization of membrane proteins, and help to keep structural integrity and activity. This 50x detergent solution is sterile-filtered and contains no other preservatives.
  • Application
    The 50x Digitonin solution is INDISPENSABLE to maintain solubilization and activity of membrane proteins, and should be used together with membrane proteins to dilute membrane proteins or antibodies, to prepare running buffer in SPR or BLI assay, etc. The 50x Digitonin solution should be diluted with assay buffer solutions to prepare 1x working solution just before experiment.
  • Storage
    Store this solution at -70℃, and protect from light. If precipitation occurs, filter to clarify before use. This Solution is stable after storage at -70℃ no more than 3 months upon receipt.

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