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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 87.1 kDa. The protein migrates as 230 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Glypican 3 (R355A,R358A) Protein, Fc Tag, premium grade on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human Glypican 3 (R355A,R358A) Protein, Fc Tag, premium grade (Cat. No. GP3-H5255) is more than 90% and the molecular weight of this protein is around 170-210 kDa verified by SEC-MALS.
Flow cytometric analysis of Anti-GPC3 CAR-293 staining with Human Glypican 3 (R355A,R358A) Protein, Fc Tag, premium grade (Cat. No. GP3-H5255) (2e5 of anti-GPC3 CAR-293 cells were stained with 100 μL of 3 μg/mL of Human Glypican 3, Fc Tag), compared with negative control protein, washed and then followed by PE anti-human IgG Fc Recombinant Antibody and analyzed with FACS (QC tested).
Immobilized Human Glypican 3 (R355A,R358A) Protein, Fc Tag, premium grade (Cat. No. GP3-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 0.1-6 ng/mL (QC tested).
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Organoid Toolbox is a collection of organoid solutions including ready-to-use organoids, organoid differentiation kits, and a variety of services to accelerate the progress of your drug development project.
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GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
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