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Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag (HPLC-verified)

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  • Source
    Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag(GIE-V5283) is expressed from human 293 cells (HEK293). It contains AA Leu 21-Leu 274 & Ser 31 - Ala 546 (Accession # Q77NN4 & Q9J3M8).
    Predicted N-terminus: Leu 21 & Ser 31
  • Molecular Characterization
    Glycoprotein I & Glycoprotein E/gI & gE (VZV) Structure

    This protein carries a polyhistidine tag and a twin strep tag at the C-terminus. The protein has a calculated MW of 30.1 kDa & 61.7 kDa. The protein migrates as 35-42 kDa and 60-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Glycoprotein I & Glycoprotein E/gI & gE (VZV) SDS-PAGE

Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-HPLC
Glycoprotein I & Glycoprotein E/gI & gE (VZV) SEC-HPLC

The purity of Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag (Cat. No. GIE-V5283) was greater than 85% as determined by SEC-HPLC.

Bioactivity-ELISA
 Glycoprotein I & Glycoprotein E/gI & gE (VZV) ELISA

Immobilized Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag (Cat. No. GIE-V5283) at 1 μg/mL (100 μL/well) can bind Anti-Varicella-Zoster Virus Antibody with a linear range of 0.1-1 ng/mL (QC tested).

  • Background
    Varicella-zoster virus (VZV) is the alphaherpesvirus that causes chicken pox (varicella) and shingles (zoster). The two VZV glycoproteins gE and gI form a heterodimer that mediates efficient cell-to-cell spread.
  • Clinical and Translational Updates

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