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Your Position: Home > Protein > IgG1 Fc > IG1-HC2H3

Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F) Protein, His Tag-Cy5

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  • Synonym
    IgG1
  • Source
    Human IgG1 Fc Protein, His Tag-Cy5(IG1-HC2H3) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # P01857-1 (C103S, M135Y, S137T, T139E, H316K, N317F)).
    Predicted N-terminus: Glu 99
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.1 kDa.

  • Conjugate

    Cy5

    Excitation Wavelength: 651 nm

    Emission Wavelength: 670 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with Cy5 using standard chemical labeling method. The residual Cy5 is removed by molecular sieve treatment during purification process.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-SPR
 IgG1 Fc SPR

Human IgG1 Fc Protein, His Tag-Cy5 (IG1-HC2H3) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) with an affinity constant of 8.27 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-FACS
 IgG1 Fc FACS

Flow cytometric analysis of HEK293/Human FcRn (FCGRT & B2M) Stable Cell staining with Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F) Protein, His Tag-Cy5 (Cat. No. IG1-HC2H3) at 1:50 dilution(2 μL of the stock solution corresponds to labeling of 2e5 cells in a final volume of 100 µL) , compared with negative control protein. Cy5 signal was used to evaluate the binding activity (QC tested).

  • Background
    Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
  • Clinical and Translational Updates

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Price(USD) : $1980.00

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