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This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 20.2 kDa. The protein migrates as 28-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human NKG2D Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human NKG2D Protein, Avitag,His Tag (Cat. No. NKD-H82Q3) is more than 85% and the molecular weight of this protein is around 36-46 kDa verified by SEC-MALS.
Immobilized Human MICA, Fc Tag (Cat. No. MIA-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human NKG2D Protein, Avitag,His Tag (Cat. No. NKD-H82Q3) with a linear range of 5-156 ng/mL (QC tested).
Immobilized Biotinylated Human NKG2D Protein, Avitag,His Tag (Cat. No. NKD-H82Q3) at 1 μg/mL (100 μL/well) on streptavidin (STN-N5116) precoated (0.5 μg/well) plate can bind Human MICA, Fc Tag (Cat. No. MIA-H5253) with a linear range of 0.05-0.8 ng/mL (Routinely tested).
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