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Use the kit after you extract residual DNA from test samples. For achieving the better DNA recovery, it is recommended to use the resDetect™ resDNA Sample Preparation Kit (Magnetic Beads) (Cat. No. OPA-R005) in combination.
The kit is used for quantitative detection of residual E1A DNA in biopharmaceutical production.
For use in quality control/manufacturing process only.
It is for research use only.
Figure 1. High sensitivity and broad dynamic range using the E1A resDNA Quantitative Kit. (A) Typical analysis results obtained with Standard 1 (4×10⁶ copies/µL) to 6 (4×10 copies/µL). (B) The standard curve of the 10-fold dilution series. PCR efficiency should be 90-110%.
To evaluate the specificity of assay using E1A resDNA Quantitation Kit, no template samples spiked with unrelated DNA were tested. Results shown in the following Table. Unrelated DNA (E. coli, CHO, Vero, and Pichia yeast) were not detected in assay, and the value of MDCK was outside the range of standard curve (4×10⁶ copies/µL to 4×10 copies/µL).
To evaluate the recovery of assay using E1A resDNA Quantitation Kit, three different concentrations of DNA samples were tested. Results shown in the following Table. All samples had recoveries between 95%-104%.
| ID | Components | Size |
| OPA-R008-01 | E1A Primer & Probe Mix | 550 μL |
| OPA-R008-02 | 2×qPCR Master Mix | 1.6 mL |
| OPA-R008-03 | Linearize DNA Control 4×10⁸ copies/μL | 50 μL |
| OPA-R008-04 | DNA Dilution Buffer | 1.5 mL×3 |
Price(USD) : $1430.00
Price(USD) :
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