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Your Position: Home > Protein > TROP-2 > TR2-R82F6

Biotinylated Rhesus macaque TROP-2 / TACSTD2 Protein, Fc,Avitag™ (MALS verified)

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  • Synonym
    TACSTD2,GA733-1,M1S1,TROP2
  • Source
    Biotinylated Rhesus macaque TROP-2 Protein, Fc,Avitag(TR2-R82F6) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 274 (Accession # XP_001114599.1).
    Predicted N-terminus: Gln 31
  • Molecular Characterization
    TROP-2 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 55.6 kDa. The protein migrates as 63-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM Nacl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TROP-2 SDS-PAGE

Biotinylated Rhesus macaque TROP-2 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
TROP-2 MALS images

The purity of Biotinylated Rhesus macaque TROP-2 Protein, Fc,Avitag (Cat. No. TR2-R82F6) is more than 85% and the molecular weight of this protein is around 118-138 kDa verified by SEC-MALS.

Bioactivity-ELISA
 TROP-2 ELISA

Immobilized Mouse Monoclonal Antibody Against Human TROP-2 Protein,Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Rhesus macaque TROP-2 Protein, Fc,Avitag (Cat. No. TR2-R82F6) with a linear range of 0.1-8 ng/mL (QC tested).

 TROP-2 ELISA

Immobilized Biotinylated Rhesus macaque TROP-2 Protein, Fc,Avitag (Cat. No. TR2-R82F6) at 1 μg/mL (100 μL/well on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Monoclonal Antibody Against Human TROP-2 Protein,Mouse IgG1 with a linear range of 0.1-8 ng/mL (Routinely tested).

  • Background
    TROP-2 is a single-copy gene in human cells, and encodes a type-1 transmembrane glycoprotein which is over-expressed in various malignancies, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1. TROP-2 is related to epithelial cell adhesion molecule (EpCAM), also called TROP-1, gp40, and KSA. Trop-1 and Trop-2 are homologous to serum IGF-II-binding proteins and appear as signal transducers. Thus, they likely represent novel cell-surface receptors and may play a role in regulating the growth of carcinoma cells.
  • Clinical and Translational Updates

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