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Your Position: Home > Antibody > FMC63 > CD9-BV4324b

Biotinylated FMC63 scFv, Fc,Avitag™ (MALS verified)

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  • Source
    Biotinylated FMC63 scFv, Fc, Avitag is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain.
  • Specificity
    Specifically recognizes the antigen-recognition domain of CD19-derived CARs.
  • Application
    Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) ADA Expression).
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20 to -70°C for 12 months in lyophilized state from date of receipt;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FMC63 SDS-PAGE

Biotinylated FMC63 scFv, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
FMC63 MALS images

The purity of Biotinylated FMC63 scFv, Fc,Avitag (Cat. No. CD9-BV4324b) is more than 90% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.

Bioactivity-Elisa
 FMC63 ELISA

Immobilized Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a at 1 μg/mL, add increasing concentrations of Rabbit Anti-Mouse FMC63 Polyclonal Antibody (Cat. No. FM3-S93) and then add Biotinylated FMC63 scFv, Fc,Avitag (Cat. No. CD9-BV4324b) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 78 ng/mL (QC tested).

  • Background
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
  • Clinical and Translational Updates

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Price(USD) : $1200.00

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