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Your Position: Home > Protein > CAS9 > CA9-S5149

GENPower™ NLS-Cas9 Nuclease, premium grade

GMP version GMP-CA9S18 is now available for seamless transition.

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  • Features

    High cleavage activity in different cell line
    High purity (>95% SDS-PAGE / SEC-MALS)
    Animal-Free materials
    Batch-to-batch consistency
    Stringent quality control tests

  • Product Details
    GENPower™ NLS-Cas9 Nuclease is a recombinant Streptococcus pyogenes Cas9 protein purified from Escherichia coli for CRISPR-based genome editing. The introduction of nuclear localization signals (NLS) can help Cas9 enter the nucleus, increasing the chance of genomic DNA cleavage.
  • Concentration
    10 mg/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Host Cell Protein
    ≤10 ng/mg of protein tested by ELISA.
  • Host Cell DNA
    ≤1 ng/mg of protein tested by qPCR.
  • Sterility
    Negative
  • Mycoplasma
    Negative.
  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 300 mM NaCl, 0.1 mM EDTA, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt;
    2. -20°C for 18 months under sterile conditions.
SDS-PAGE
CAS9 SDS-PAGE

The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CAS9 MALS images

The purity of GENPower™ NLS-Cas9 Nuclease, premium grade (Cat. No. CA9-S5149) is more than 95% and the molecular weight of this protein is around 140-180 kDa verified by SEC-MALS.

Bioactivity
 CAS9 ENZYME

GENPower™ NLS-Cas9 Nuclease, premium grade is evaluated in an in vitro DNA cleavage assay on a DNA fragment containing the target sequence. The activity of the GENPower™ NLS-Cas9 Nuclease, premium grade is greater than 90% (QC tested).

 CAS9 ENZYME

The cleavage activity of GENPower™ NLS-Cas9 Nuclease, premium grade in vitro.

 CAS9 ENZYME

The cleavage activity of GENPower™ NLS-Cas9 Nuclease, premium grade in cell line.

 CAS9 ENZYME

The cleavage activity of GENPower™ NLS-Cas9 Nuclease, premium grade in human primary T cells.

  • Clinical and Translational Updates

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DMF (Drug Master File)

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