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Your Position: Home > Protein > CD3E & CD3G > CDG-H5253

Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

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  • Synonym
    CD3 epsilon & CD3 gamma,CD3E & CD3G
  • Source
    Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag(CDG-H5253) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1 (CD3E) & AAI13831 (CD3G)).
    Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)
  • Molecular Characterization
    CD3E & CD3G Structure

    Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 38.3 kDa (CD3E) and 36.9 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus. As a result of glycosylation, the protein migrates as 43-55 kDa under reducing (R) condition, and 90-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD3E & CD3G SDS-PAGE

Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD3E & CD3G MALS images

The purity of Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) is more than 90% and the molecular weight of this protein is around 80-90 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD3E & CD3G ELISA

Immobilized Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with a linear range of 0.8-3 ng/mL (QC tested).

 CD3E & CD3G ELISA

Immobilized Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) at 1 μg/mL (100 μL/well) can bind Mouse Anti Human CD3 with a linear range of 0.8-2 ng/mL (Routinely tested).

 CD3E & CD3G ELISA

Immobilized Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) at 1 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.8-3 ng/mL (Routinely tested).

 CD3E & CD3G ELISA

Immobilized Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) at 1 μg/mL (100 μL/well) can bind Bispecific BCMA×CD3 T cell-engaging Antibody with a linear range of 0.8-3 ng/mL (Routinely tested).

  • Background
    T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • Clinical and Translational Updates

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