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ID | Components | Size |
RAS023-C01 | Pre-coated SARS-CoV-2 Spike Protein Microplate | 1 plate |
RAS023-C02 | Anti-SARS-CoV-2 Antibody (Control, Mouse IgG ) | 100 μL |
RAS023-C03 | HRP-Conjugated Antibody | 10 μg |
RAS023-C04 | 10 x Washing Buffer | 50 mL |
RAS023-C05 | Dilution Buffer | 50 mL |
RAS023-C06 | Substrate Solution | 12 mL |
RAS023-C07 | Stop Solution | 7 mL |
RAS023-C08 | SARS-CoV-2 Antibody Positive Control | 100 μL |
RAS023-C09 | SARS-CoV-2 Antibody Negative Control | 100 μL |
ACROBiosystems, a leading life science company dedicated to aid the global COVID-19 response,developed different kits suitable for serum antibody detection in different species (Mouse, Monkeys, humans) to meet the needs of preclinical and clinical experiments.
This kit is developed for detection of Mouse Anti-SARS-CoV-2 antibody (IgG) in serum in vitro.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 5 simple steps:
a) Add your diluted Samples and Control to the plate.
b) Add diluted Secondary Antibody HRP-Goat-Anti-Mouse IgG to the plate.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by adding diluted acid.
e) To remove background noise prior to statistical analysis, absorbance (OD) is calculated by substracting the value at 450 nm with the value at 630 nm. The OD Value reflects the amount of antibody bound.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Protein at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG in 1:100 mouse serum. Detection was performed using HRP-Anti-mouse IgG antibody with sensitivity of 20 ng/mL (QC tested).
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