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This protein carries no "tag". The protein has a calculated MW of 24.8 kDa. The protein migrates as 28-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Rabbit IgG Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) is more than 90% and the molecular weight of this protein is around 52-66 kDa verified by SEC-MALS.
Immobilized Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.3-5 ng/mL (QC tested).
Immobilized Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) with a linear range of 0.02-0.625 μg/mL (Routinely tested).
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