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Mouse
Mouse IgG1 | Kappa
Unconjugated
Hybridoma Monoclonal
Virus
This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
| Application | Recommended Usage |
| ELISA | 0.15-20 ng/mL |
>95% as determined by SDS-PAGE.
Protein A purified/ Protein G purified
Supplied as 0.2 μm filtered solution in PBS, pH7.4 .
Contact us for customized product form or formulation.
This product is supplied and shipped with blue ice, please inquire the shipping cost.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H1) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (Cat. No. S1N-S58) with a linear range of 0.15-2.5 ng/mL (QC tested).
Detection SARS-CoV-2 S protein RBD by Sandwich ELISA Assay.
Immobilized Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (Cat. No. S1N-S58) at 1 μg/mL (100 μL/well) can bind S protein RBD. And then add Anti-SARS-CoV-2 Spike S1 Antibody at 1:5000. Detection was performed using high sensitivity HRP-conjugated streptavidin with sensitivity of 5 pg/mL (Routinely tested).
Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (Cat. No. S1N-S58) captured on CM5 chip via Anti-mouse antibodies surface can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 0.246 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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