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Your Position: Home > Antibody > Spike NTD > SPD-M121

Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 (AM121)

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  • Source
    Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 (AM121) (SPD-M121) is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions.
  • Clone

    AM121

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Unconjugated

  • Reactivity

    Virus

  • Specificity

    This product is a specific antibody against SARS-CoV-2 S1 protein NTD.

  • Application
    ApplicationRecommended Usage
    ELISA0.2-10 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike NTD SDS-PAGE

Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 (AM121) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 Spike NTD ELISA

Immobilized SARS-CoV-2 S1 protein NTD, His Tag (Cat. No. S1D-C52H6) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 (AM121) (Cat. No. SPD-M121) with a linear range of 0.2-2 ng/mL (QC tested).

Bioactivity-SPR
 Spike NTD SPR

Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 (AM121) (Cat. No. SPD-M121) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S1 protein NTD, His Tag (Cat. No. S1D-C52H6) with an affinity constant of 74.6 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
 Spike NTD BLI

Loaded Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 (AM121) (Cat. No. SPD-M121) on AHC Biosensor, can bind SARS-CoV-2 S1 protein NTD, His Tag (Cat. No. S1D-C52H6) with an affinity constant of 39.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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