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Your Position: Home > Protein > Fc gamma RI / CD64 > FCA-H52H1

Human Fc gamma RI / CD64 Protein, His Tag (SPR & BLI & MALS verified)

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  • Synonym
  • Source
    Human CD64, His Tag(FCA-H52H1) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Leu 281 (Accession # P12314-1).
    Predicted N-terminus: Gln 16
  • Molecular Characterization
    Fc gamma RI / CD64 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 31.8 kDa. The protein migrates as 45-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
Fc gamma RI / CD64 SDS-PAGE

Human CD64, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Fc gamma RI / CD64 MALS images

The purity of Human CD64, His Tag (Cat. No. FCA-H52H1) is more than 90% and the molecular weight of this protein is around 44-60 kDa verified by SEC-MALS.

 Fc gamma RI / CD64 ELISA

Immobilized Ipilimumab at 1 μg/mL (100 μL/well) can bind Human CD64, His Tag (Cat. No. FCA-H52H1) with a linear range of 2-8 ng/mL (QC tested).

 Fc gamma RI / CD64 SPR

Human CD64, His Tag (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herceptin® with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K) (QC tested).

 Fc gamma RI / CD64 SPR

Herceptin captured on Protein A Chip can bind Human CD64, His Tag (Cat. No. FCA-H52H1) with an affinity constant of 0.108 nM as determined in SPR assay (Biacore 8K) (Routinely tested).

 Fc gamma RI / CD64 BLI

Loaded Human CD64, His Tag (Cat. No. FCA-H52H1) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 6.98 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Fc gamma RI / CD64 BLI

Loaded Herceptin on Protein A Biosensor, can bind Human CD64, His Tag (Cat. No. FCA-H52H1) with an affinity constant of 0.297 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Fc gamma RI / CD64 BLI

Loaded Herceptin on FAB2G Biosensor, can bind Human CD64, His Tag (Cat. No. FCA-H52H1) with an affinity constant of 0.244 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.
    High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
  • Clinical and Translational Updates

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  • Latest Research Phase:Discontinued

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