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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 47.3 kDa. The protein migrates as 60-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Fc epsilon RI alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human Fc epsilon RI alpha, Fc Tag (Cat. No. FCA-H5259) is more than 90% and the molecular weight of this protein is around 125-135 kDa verified by SEC-MALS.
Human Fc epsilon RI alpha, Fc Tag (Cat. No. FCA-H5259) immobilized on CM5 Chip can bind Human IgE Fc, His Tag (Cat. No. IGE-H52H9) with an affinity constant of 0.585 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Human Fc epsilon RI alpha, Fc Tag (Cat. No. FCA-H5259) on AHC Biosensor, can bind Immunoglobulin E, Human Plasma with an affinity constant of 0.07 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
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