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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 23.9 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Cynomolgus CD32a, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Cynomolgus CD32a, His,Avitag (Cat. No. CDA-C82E5) is more than 85% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.
Captured Biotinylated Cynomolgus CD32a, His,Avitag (Cat. No. CDA-C82E5) on Biotin CAP - Series S sensor Chip can bind MabThera® (Rituximab) with an affinity constant of 7.55 μM as determined in a SPR assay (Biacore T200) (QC tested).
Loaded Biotinylated Cynomolgus CD32a, His,Avitag (Cat. No. CDA-C82E5) on SA Biosensor, can bind MabThera® (Rituximab) with an affinity constant of 2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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