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This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 62.4 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Nectin-2, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) at 5 μg/mL (100 μL/well) can bind Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) with a linear range of 2-78 ng/mL (QC tested).
Immobilized Biotinylated Human PVRIG, His,Avitag (Cat. No. PVG-H82E3) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, bind Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) with a linear range of 0.1-5 ng/mL (Routinely tested).
Loaded Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) on Protein A Biosensor, can bind Human PVRIG, His Tag (Cat. No. PVG-H52H4) with an affinity constant of 1.33 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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