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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 26.4 kDa respectively. The protein migrates as 33 kDa, 43 kDa and 70-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human Glypican 3, His,Avitag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human Glypican 3, His,Avitag, premium grade (Cat. No. GP3-H82E5) is more than 90% and the molecular weight of this protein is around 65-75 kDa verified by SEC-MALS.
2e5 of anti-GPC3 CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Human Glypican 3, His,Avitag, premium grade (Cat. No. GP3-H82E5) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Immobilized Monoclonal Anti-Human GPC3 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human Glypican 3, His,Avitag, premium grade (Cat. No. GP3-H82E5) with a linear range of 0.2-3 ng/mL (QC tested).
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