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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 34.2 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CD64, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) is more than 90% and the molecular weight of this protein is around 46-66 kDa verified by SEC-MALS.
Immobilized Ipilimumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.1-3 ng/mL (QC tested).
Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) captured on Biotin CAP - Series S sensor Chip can bind Anti-Her2 antibody, Human IgG1 | Human Kappa with an affinity constant of 0.435 nM as determined in a SPR assay (Biacore T200) (QC tested).
Loaded Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) on SA Biosensor, can bind Herceptin with an affinity constant of 0.515 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) on SA Biosensor, can bind Human IgG Fc, His Tag (Cat. No. IG1-H5225) with an affinity constant of 1.60 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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