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This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.
The protein has a calculated MW of 44.9 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human BAFF Protein, Avitag,Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) is more than 85% and the molecular weight of this protein is around 280-320 kDa verified by SEC-MALS.
Immobilized Human BCMA, Fc Tag (Cat. No. BC7-H5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) with a linear range of 1.95-15.6 ng/mL (QC tested).
Immobilized Human TACI Protein, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) with a linear range of 0.2-6 ng/mL (Routinely tested).
Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258): Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.8129 μg/mL (Routinely tested).
Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR, Fc Tag (Cat. No. BAR-H5257): Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.4692 μg/mL (Routinely tested).
Immobilized Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) on SA Chip can bind Human TACI Protein, Fc Tag with an affinity constant of 0.988 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Immobilized Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) on SA Chip can bind Human BAFFR, Fc Tag (Cat. No. BAR-H5257) with an affinity constant of 26.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) on SA Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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