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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.2 kDa. The protein migrates as 18-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus / Rhesus macaque TIGIT, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Cynomolgus / Rhesus macaque TIGIT, His Tag (Cat. No. TIT-C5223) was greater than 90% as determined by SEC-HPLC.
Immobilized Cynomolgus / Rhesus macaque TIGIT, His Tag (Cat. No. TIT-C5223) at 5 μg/mL (100 μL/well) can bind Human CD155, Fc Tag (Cat. No. CD5-H5251) with a linear range of 0.078-2.5 μg/mL (QC tested).
Loaded Human CD155, Fc Tag (Cat. No. CD5-H5251) on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque TIGIT, His Tag (Cat. No. TIT-C5223) with an affinity constant of 0.61 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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