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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 64.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Nectin-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human Nectin-1, His Tag (Cat. No. PV1-H5223) at 2 μg/mL (100 μL/well) can bind Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) with a linear range of 5-78 ng/mL (QC tested).
Immobilized Human TIGIT, His Tag (Cat. No. TIT-H52H5) at 1 μg/mL (100 μL/well) can bind Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) with a linear range of 0.078-5 μg/mL (Routinely tested).
Loaded Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.732 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) on Protein A Biosensor, can bind Human Nectin-1, His Tag (Cat. No. PV1-H5223) with an affinity constant of 0.52 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human Nectin-1, His Tag (Cat. No. PV1-H5223) on HIS1K Biosensor, can bind Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) with an affinity constant of 0.14 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Explore our catalog of therapeutic antibody solutions to find the right products for you! We are dedicated to delivering solutions designed to help you drive innovation and push the boundaries of what therapeutic antibodies can be.
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Organoid Toolbox is a collection of organoid solutions including ready-to-use organoids, organoid differentiation kits, and a variety of services to accelerate the progress of your drug development project.
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GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
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