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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.9 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IgG Fc, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IgG Fc, His Tag (Cat. No. IG1-H5225) is more than 95% and the molecular weight of this protein is around 50-66 kDa verified by SEC-MALS.
Loaded Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) on SA Biosensor, can bind Human IgG Fc, His Tag (Cat. No. IG1-H5225) with an affinity constant of 1.60 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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