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ID | Components | Size |
EP101-C01 | High-bind Plate | 1 plate |
EP101-C02 | Human PD-L1 | 25 μg |
EP101-C03 | Anti-PD-1 Neutralizing Antibody | 20 μg |
EP101-C04 | Human PD-1-Biotin | 5 μg |
EP101-C05 | Streptavidin-HRP | 10 μg |
EP101-C06 | Coating Buffer | 12 mL |
EP101-C07 | 10xWashing Buffer | 50 mL |
EP101-C08 | Blocking Buffer | 50 mL |
EP101-C09 | Substrate Solution | 12 mL |
EP101-C10 | Stop Solution | 7 mL |
It is for research use only.
Your experiment will include 4 simple steps:
a) Coat the plate with human PD-L1.
b) Add your molecule of interest to the tests.
c) Add human PD-1-Biotin to bind the coated human PD-L1.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit PD-1 : PD-L1 binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF PD-1-PD-L1 BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY (CATALOG # EP101-C03) MEASURED USING THE PD-1 [BIOTINYLATED] : PD-L1 INHIBITOR SCREENING ELISA ASSAY PAIR (CATALOG # EP-101).
Anti-PD-1 neutralizing antibody was diluted from 10 μg/mL to 0.078 μg/ mL (69.628 nM to 0.544 nM) and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin. Assay was performed according to the protocol in PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $600.00
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