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Your Position: Home > Kits > EGF > CEA-C080

ClinMax™ Human EGF ELISA Kit

For research use only.

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Product Details
Assay TypeSandwich-ELISA
Standard Curve Range62.5 pg/mL-8000 pg/mL
Assay Time2 hr 45 min
Suitable Sample TypeFor the quantitative determination of human EGF in Cell Culture Supernatants, Plasma, Serum.
Sample volume50 uL
Materials Provided
CEA080-C01Pre-coated Anti-EGF Antibody Microplate1 plate
CEA080-C02Human EGF Standard7.5 ug×2
CEA080-C03Biotin-Anti-EGF Antibody Con. Solution100 μL
CEA080-C04Biotin-Antibody Dilution Buffer8 mL
CEA080-C05Streptavidin-HRP Con. Solution500 μL
CEA080-C06Streptavidin-HRP Dilution Buffer15 mL
CEA080-C0720× Washing Buffer50 mL
CEA080-C08Sample Dilution Buffer15 mL×2
CEA080-C09Substrate Solution12 mL
CEA080-C10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human EGF that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-like facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of human EGF in serum, plasma and cell culture supernates.

    It is for research use only.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     EGF Assay Principles
Typical Data Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of EGF is less than 35 pg/mL.

Intra-Assay Statistics

Ten replicates of each of 3 samples containing different EGF concentrations were tested in one assay. Acceptable criteria: CV<10%.

Inter-Assay Statistics

Three samples containing different concentrations of EGF were tested in independent assays. Acceptable criteria: CV<15%.


Recombinant EGF was spiked into 3 human serum samples, and then analyzed. The average recovery of EGF for serum samples is 105%.

  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $498.00

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  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Phase 2 Clinical

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