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ClinMax™ Human IFN-γ ELISA Kit, PRO

For research use only.

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Product Details
Assay TypeSandwich-ELISA
Regulatory StatusRUO
Sensitivity<4.0 pg/mL
Standard Curve Range7.18 pg/mL-1000 pg/mL
Assay Time2 hr
Suitable Sample TypeFor the quantitative determination of human IFN-γ in Plasma and Serum.
Sample volume50 μL
Materials Provided
CEA006-C01Pre-coated Anti-IFN-γ Antibody Microplate1 plate
CEA006-C02IFN-γ Calibrator15 μg×2
CEA006-C03Biotin-Anti-IFN-γ Antibody Concentrated Solution100 μL
CEA006-C04Biotin Antibody Dilution Buffer8 mL
CEA006-C05IFN-γ Streptavidin-HRP Concentrated Solution0.5 mL
CEA006-C06Streptavidin-HRP Dilution Buffer15 mL
CEA006-C0720× Washing Buffer50 mL
CEA006-C08Sample Dilution Buffer15 mL×2
CEA006-C09Substrate Solution12 mL
CEA006-C10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IFN-gamma that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    Results are obtained by Log-Log Linear regression equation are used to draw the standard curve and calculate the sample concentration. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human IFN-γ concentrations.

    It has been calibrated against a highly purified human IFN-γ and is evaluated with standard from NIBSC/WHO. Reference Reagent INTERFERON GAMMA (Human, rDNA derived) NIBSC code: 87/586.

    It is for research use only.

  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
     IFN-gamma Assay Principles
Typical Data Please refer to DS document for The assay protocol.

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA-C006 is less than 4.0 pg/mL.

Intra-Assay Statistics

Ten replicates of each of five samples containing different IFN-γ concentrations were tested in one assay, Intra-Assay Precision CV<10%.

Inter-Assay Statistics

Five samples containing different concentrations of IFN-γ were tested in three independent assays, Inter-Assay Precision CV<15%.


Recombinant IFN-γ(8000,6000,4000pg/mL)was spiked into 5 human serum samples, and then analyzed. On average, 98.28% of IFN-γ was recovered from serum samples.

  • Background: IFN-gamma
    Interferon-γ, IFN-γ, Interferon Gamma
    Interferon-γ is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
    IFN-γ is capable of orchestrating numerous protective functions to heighten immune responses in infections and cancers. It can exhibit its immunomodulatory effects by enhancing antigen processing and presentation, increasing leukocyte trafficking, inducing an anti-viral state, boosting the anti-microbial functions and affecting cellular proliferation and apoptosis.
  • Clinical and Translational Updates

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