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Your Position: Home > Kits > VEGF-A > CEA-C068

ClinMax™ Human VEGF ELISA Kit

For research use only.

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Product Details
Assay TypeSandwich-ELISA
AnalyteVEGF
Format96T
ReactivityHuman
Sensitivity<7pg/mL
Standard Curve Range31.25 pg/mL-2000 pg/mL
Assay Time3 hr 10 min
Suitable Sample TypeFor the quantitative determination of human VEGF in Cell Culture Supernatants, Plasma, Serum.
Sample volume100 uL
Materials Provided
IDComponentsSize
CEA068-C01Pre-coated Anti-VEGF Antibody Microplate1 plate
CEA068-C02Human VEGF Standard2000 pg×2
CEA068-C03Biotin-Anti-VEGF Antibody Con. Solution120 μL
CEA068-C04Biotin-Antibody Dilution Buffer15 mL
CEA068-C05Streptavidin-HRP Con. Solution120 μL
CEA068-C06Streptavidin-HRP Dilution Buffer15 mL
CEA068-C0720× Washing Buffer25 mL
CEA068-C08Sample Dilution Buffer15 mL×2
CEA068-C09Substrate Solution15 mL
CEA068-C10Stop Solution15 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human VEGF/VEGF-A that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-like facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of human VEGF/VEGF-A in serum, plasma and cell culture supernates.

    It is for research use only.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     VEGF-A Assay Principles
Typical Data Please refer to Ds document for the assay protocol.
 VEGF-A TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of VEGF is less than 7 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 3 samples containing different VEGF concentrations were tested in one assay. Acceptable criteria: CV<10%.

 VEGF-A INTRA-ASSAY STATISTICS
Inter-Assay Statistics

3 samples containing different concentrations of VEGF were tested in independent assays. Acceptable criteria: CV<15%.

 VEGF-A INTER-ASSAY STATISTICS
Recovery

Recombinant VEGF was spiked into 3 human serum samples, and then analyzed. The average recovery of VEGF for serum samples is 85.87%.

 VEGF-A RECOVERY
  • Background: VEGF-A
    The vascular endothelial growth factor (VEGF) family of cytokines plays a key role in vasculogenesis, angiogenesis, and lymphangiogenesis. VEGF-A is the main member of this family, alongside placental growth factor (PlGF), VEGF-B/C/D in mammals, and VEGF-E/F in other organisms.
  • Clinical and Translational Updates

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Price(USD) : $549.00

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