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Your Position: Home > Protein > DLL3 > DL3-H525x

Human DLL3 Protein, Llama IgG2b Fc Tag

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  • Synonym
    Delta3,delta-like 3 (Drosophila),delta-like protein 3,DLL3,Pudgy,SCDO1,SCDO1delta3
  • Source
    Human DLL3 Protein, Llama IgG2b Fc Tag(DL3-H525x) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Leu 492 (Accession # Q9NYJ7-1).
    Predicted N-terminus: Ala 27
  • Molecular Characterization
    DLL3 Structure

    This protein carries a llama IgG2b Fc tag at the C-terminus.

    The protein has a calculated MW of 76.6 kDa. The protein migrates as 75-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.2 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
DLL3 SDS-PAGE

Human DLL3 Protein, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 DLL3 ELISA

Immobilized Human DLL3 Protein, Llama IgG2b Fc Tag (Cat. No. DL3-H525x) at 1 μg/mL (100 μL/well) can bind Anti-DLL3 Antibody (specific Binding DSL of DLL3), Human IgG1 (Cat. No. AM186) with a linear range of 0.39-6.25 ng/mL (QC tested).

 DLL3 ELISA

Immobilized Human DLL3, Llama IgG2b Fc Tag (Cat. No. DL3-H525x) at 1 μg/mL (100 μL/well) can bind Anti-DLL3 Antibody (specific Binding EGFs of DLL3), Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).

  • Background
    Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands.May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. DLL3 protein is expressed on the surface of tumor cells but not in normal adult tissues.
  • Clinical and Translational Updates

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