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Product | Size | Amount |
Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed | 2.5 mg | 2.5 × 10⁷ beads |
10 mg | 1.0 × 10⁸ beads |
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Fig.1. Activation of the purified human T Cells.The human T cells were stimulated with ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade(Cat. No. MBS-C001) for 24hrs, and the activation was assessed by measuring expression of both activation markers CD25 and CD69 expression on the T cells surface by stanning with PE labeled anti-human CD25 antibody and FITC labeled anti-human CD69 antibody respectively (QC tested).
Fig.2. Purified human T Cells expansion.The human T cells were labeled with carboxy fluorescein succinimidyl ester (CFSE) and stimulated with ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade(Cat. No. MBS-C001), and then the proliferation of the T cells was assessed with CFSE dilution assay by flow cytometry on day 5 after stimulation (QC tested).
Activation of the purified human T Cells. The purified human T cells were activated using Human T cell Activation/Expansion CD3/CD28 Beads, (ACRO, Cat. No. GMP-MBS001/MBS-C001) respectively for 24 hours with CTS Optimizer Medium. Cells were fluorescently stained using PE labeled anti-human CD25 antibody and labeled FITC anti-human CD69 antibody and analyzed by flow cytometry.
Expansion of the human CD3+T cells. Human T cells using ACROBiosystems CD3/CD28 Beads (ACRO, Cat. No. GMP-MBS001/MBS-C001) were expanded under two different medium, respectively. Expansion was performed for two weeks, showing that ACROBiosystems’ GMP and PG beads showing similar proliferative abilities.
PD-1 expression of the activated human T Cells. The purified human T cells were stimulated using Human T cell Activation/Expansion CD3/CD28 Beads at a ratio of 1:1 beads-to-cells. Cells were expanded in T cell culture medium supplemented with 4ng/mL of rhIL-2 Protein. Activated T cells were expanded for up to 8 days with low PD-1 expression.
Price(USD) : $200.00
Price(USD) : $550.00
Price(USD) : $1980.00
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