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Your Position: Home > Immune Checkpoint Proteins > SIRP alpha > SIA-M82E2

Biotinylated Mouse SIRP alpha / CD172a Protein, His,Avitag™ (MALS verified)

  • Synonym
    SIRP alpha,SHPS1,SIRPA,CD172A,BIT,SHPS-1,MFR,MYD-1,MYD1,PTPNS1,SIRPalpha,p84,SIRP-alpha,CD172
  • Source
    Biotinylated Mouse SIRP alpha, His,Avitag (SIA-M82E2) is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Asn 373 (Accession # P97797-1).
    Predicted N-terminus: Lys 32
  • Molecular Characterization
    Online(Lys 32 - Asn 373) P97797-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 41.5 kDa. The protein migrates as 55-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse SIRP alpha, His,Avitag (Cat. No. SIA-M82E2) SDS-PAGE gel

Biotinylated Mouse SIRP alpha, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Mouse SIRP alpha, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Mouse SIRP alpha, His,Avitag (Cat. No. SIA-M82E2) was more than 90% and the molecular weight of this protein is around 63-78kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Mouse SIRP alpha, His,AvitagBiotinylated Mouse SIRP alpha, His,Avitag (Cat. No. SIA-M82E2) ELISA bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Mouse SIRP alpha, His,Avitag (Cat. No. SIA-M82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-M525a) with a linear range of 0.2-5 ng/mL (QC tested).

  • Background
    Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:4 Details
  • Latest Research Phase:Approved

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