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Your Position: Home > Protein > Mesothelin > MSN-HR2P4

RFP Fusion Human Mesothelin / MSLN (296-580) Protein, His Tag

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  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    RFP Fusion Human Mesothelin (296-580), His Tag(MSN-HR2P4) is expressed from human 293 cells (HEK293). It contains AA Glu 296-Gly 580 (Accession # AAH09272.1).
    Predicted N-terminus: Glu 296
  • Molecular Characterization
    Mesothelin Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 62.0 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    RFP

    Excitation source: 561 nm spectral line, Yellow Green Laser

    Excitation Wavelength: 561 nm

    Emission Wavelength: 586/15 nm

  • Application
    Flow cytometry (E.g. Evaluation of anti-MSLN CAR expression), fluorescence microscopy, etc.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mesothelin SDS-PAGE

RFP Fusion Human Mesothelin (296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 Mesothelin ELISA

Immobilized Anti-Human MSLN Mab, Human IgG1 at 10 μg/mL (100 μL/well) can bind RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4) with a linear range of 0.078-0.625 μg/mL (QC tested).

Bioactivity-SPR
 Mesothelin SPR

Anti-human MSLN mAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface, can bind RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4) with an affinity constant of 0.304 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-FACS
 Mesothelin FACS

2e5 of the MSLN-CAR-293 cells were stained with 100 μL of 3 μg/mL of RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4). Non-transfected 293 cells were used as negative control (QC tested).

  • Background
    Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
  • Clinical and Translational Updates

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