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Your Position: Home > Protein > Glycoprotein / GP (virus) > ZE5-V5220

Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) Envelope Glycoprotein 1 (GP1) Protein, His Tag

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  • Synonym
    GP,Envelope glycoprotein,GP(1,2)
  • Source
    Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP1, His Tag(ZE5-V5220) is expressed from human 293 cells (HEK293). It contains AA Ile 33 - Arg 501 (Accession # AAQ55048.1 ).
    Predicted N-terminus: Ile 33
  • Molecular Characterization
    Glycoprotein / GP (virus) Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 52.0 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Glycoprotein / GP (virus) SDS-PAGE

Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
  • Clinical and Translational Updates

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