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This protein carries a polyhistidine tag at the C-terminus, and an N-terminal human KLK-5 propeptide (Val 23 - Arg 66). The protein has a calculated MW of 32.7 kDa. The protein migrates as 38-53 kda when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Kallikrein 1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human Kallikrein 1 Protein, His Tag (Cat. No. KL1-H52H3) is more than 90% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >7,000 pmol/min/µg (QC tested).
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